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DNA, RNA and Protein Worksheet Generator

Complementary strands, transcription, translation, and mutation classification using the NCBI genetic code. No AI, no credits.

Grades 8 – 12No account needed

Problem sets for complementary DNA strands, transcription to mRNA, translation to protein using the standard genetic code (NCBI transl_table=1, fetched and stored with provenance), reading frames, and mutation classification (silent, missense, nonsense, frameshift). Every answer is computed by two independent translation methods. No AI, so it never uses a generation credit.

What students practice

  • Biology
  • Genetics
  • Molecular biology

Live sample sheet

No account needed to view and print.
Name: ____________________Date: ____________

DNA, RNA and Protein

1. What is the mRNA sequence transcribed from the template DNA strand: 3'-TCGAGTGCTACG-5'?
2. What is the complementary DNA strand for: 5'-AGGGATCAGATA-3'?
3. What is the complementary DNA strand for: 5'-CGAACTCTTAAG-3'?
4. What is the mRNA sequence transcribed from the template DNA strand: 3'-AGATGCGATTAG-5'?
5. What is the mRNA sequence transcribed from the template DNA strand: 3'-GCTGCTAGTTTC-5'?
6. What is the complementary DNA strand for: 5'-GGCCTACAGTGG-3'?
7. What is the mRNA sequence transcribed from the template DNA strand: 3'-CGCCCCCCAACA-5'?
8. What is the mRNA sequence transcribed from the template DNA strand: 3'-CGCTCTCGTATG-5'?
Made with TeachSpark

Answer Key — DNA, RNA and Protein

1. What is the mRNA sequence transcribed from the template DNA strand: 3'-TCGAGTGCTACG-5'?
Solution:
Template DNA: 3'-TCGAGTGCTACG-5'
Transcription rules: A→U, T→A, G→C, C→G
T
→
A
→
C
→
G
→
G
→
C
→
A
→
U
→
G
→
C
→
T
→
A
→
G
→
C
→
C
→
G
→
T
→
A
→
A
→
U
→
C
→
G
→
G
→
C
mRNA: 5'-AGCUCACGAUGC-3'
Check: each base correctly transcribed (verified by code)
Answer: AGCUCACGAUGC
2. What is the complementary DNA strand for: 5'-AGGGATCAGATA-3'?
Solution:
Template: 5'-AGGGATCAGATA-3'
Base pairing rules: A↔T, G↔C
A
→
T
→
G
→
C
→
G
→
C
→
G
→
C
→
A
→
T
→
T
→
A
→
C
→
G
→
A
→
T
→
G
→
C
→
A
→
T
→
T
→
A
→
A
→
T
Complement: 5'-TCCCTAGTCTAT-3'
Check: complement of complement = original (verified by code)
Answer: TCCCTAGTCTAT
3. What is the complementary DNA strand for: 5'-CGAACTCTTAAG-3'?
Solution:
Template: 5'-CGAACTCTTAAG-3'
Base pairing rules: A↔T, G↔C
C
→
G
→
G
→
C
→
A
→
T
→
A
→
T
→
C
→
G
→
T
→
A
→
C
→
G
→
T
→
A
→
T
→
A
→
A
→
T
→
A
→
T
→
G
→
C
Complement: 5'-GCTTGAGAATTC-3'
Check: complement of complement = original (verified by code)
Answer: GCTTGAGAATTC
4. What is the mRNA sequence transcribed from the template DNA strand: 3'-AGATGCGATTAG-5'?
Solution:
Template DNA: 3'-AGATGCGATTAG-5'
Transcription rules: A→U, T→A, G→C, C→G
A
→
U
→
G
→
C
→
A
→
U
→
T
→
A
→
G
→
C
→
C
→
G
→
G
→
C
→
A
→
U
→
T
→
A
→
T
→
A
→
A
→
U
→
G
→
C
mRNA: 5'-UCUACGCUAAUC-3'
Check: each base correctly transcribed (verified by code)
Answer: UCUACGCUAAUC
5. What is the mRNA sequence transcribed from the template DNA strand: 3'-GCTGCTAGTTTC-5'?
Solution:
Template DNA: 3'-GCTGCTAGTTTC-5'
Transcription rules: A→U, T→A, G→C, C→G
G
→
C
→
C
→
G
→
T
→
A
→
G
→
C
→
C
→
G
→
T
→
A
→
A
→
U
→
G
→
C
→
T
→
A
→
T
→
A
→
T
→
A
→
C
→
G
mRNA: 5'-CGACGAUCAAAG-3'
Check: each base correctly transcribed (verified by code)
Answer: CGACGAUCAAAG
6. What is the complementary DNA strand for: 5'-GGCCTACAGTGG-3'?
Solution:
Template: 5'-GGCCTACAGTGG-3'
Base pairing rules: A↔T, G↔C
G
→
C
→
G
→
C
→
C
→
G
→
C
→
G
→
T
→
A
→
A
→
T
→
C
→
G
→
A
→
T
→
G
→
C
→
T
→
A
→
G
→
C
→
G
→
C
Complement: 5'-CCGGATGTCACC-3'
Check: complement of complement = original (verified by code)
Answer: CCGGATGTCACC
7. What is the mRNA sequence transcribed from the template DNA strand: 3'-CGCCCCCCAACA-5'?
Solution:
Template DNA: 3'-CGCCCCCCAACA-5'
Transcription rules: A→U, T→A, G→C, C→G
C
→
G
→
G
→
C
→
C
→
G
→
C
→
G
→
C
→
G
→
C
→
G
→
C
→
G
→
C
→
G
→
A
→
U
→
A
→
U
→
C
→
G
→
A
→
U
mRNA: 5'-GCGGGGGGUUGU-3'
Check: each base correctly transcribed (verified by code)
Answer: GCGGGGGGUUGU
8. What is the mRNA sequence transcribed from the template DNA strand: 3'-CGCTCTCGTATG-5'?
Solution:
Template DNA: 3'-CGCTCTCGTATG-5'
Transcription rules: A→U, T→A, G→C, C→G
C
→
G
→
G
→
C
→
C
→
G
→
T
→
A
→
C
→
G
→
T
→
A
→
C
→
G
→
G
→
C
→
T
→
A
→
A
→
U
→
T
→
A
→
G
→
C
mRNA: 5'-GCGAGAGCAUAC-3'
Check: each base correctly transcribed (verified by code)
Answer: GCGAGAGCAUAC
Answers computed by code and double-checked by two independent methods.

Assumptions and Conventions

Assumptions:
  • The standard genetic code (NCBI transl_table=1) is used unless stated otherwise.
  • DNA sequences use the DNA alphabet (T, not U); mRNA uses U.
  • Translation starts at AUG (start codon) and stops at UAA, UAG, or UGA.
  • Reading frame: codons are read in groups of 3 from the start codon.
Conventions:
  • Course: biology
  • Difficulty: standard
Genetic code source: NCBI Taxonomy — The Standard Code (transl_table=1) (table 1), retrieved 2026-10-11.
Made with TeachSpark

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Frequently asked questions

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Can I print the DNA, RNA and Protein without signing up?

Yes. This tool computes answers by code, so you can generate, preview and print worksheet without an account. A free account adds saving and student links.

How are the answers computed?

Every answer is computed by code and verified by a second independent method. No AI is used.

What grades is the DNA, RNA and Protein for?

This tool fits Grades 8 – 12.

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Sample output, computed by code — change the options and Generate to make your own
Name: ____________________Date: ____________

DNA, RNA and Protein

1. What is the mRNA sequence transcribed from the template DNA strand: 3'-TTACCTTATTGT-5'?
2. What is the complementary DNA strand for: 5'-ACGCTCCACCTT-3'?
3. What is the mRNA sequence transcribed from the template DNA strand: 3'-CTGCTACGCAAG-5'?
4. What is the mRNA sequence transcribed from the template DNA strand: 3'-CCAAGCTTGAGA-5'?
Made with TeachSpark

Answer Key — DNA, RNA and Protein

1. What is the mRNA sequence transcribed from the template DNA strand: 3'-TTACCTTATTGT-5'?
Solution:
Template DNA: 3'-TTACCTTATTGT-5'
Transcription rules: A→U, T→A, G→C, C→G
T
→
A
→
T
→
A
→
A
→
U
→
C
→
G
→
C
→
G
→
T
→
A
→
T
→
A
→
A
→
U
→
T
→
A
→
T
→
A
→
G
→
C
→
T
→
A
mRNA: 5'-AAUGGAAUAACA-3'
Check: each base correctly transcribed (verified by code)
Answer: AAUGGAAUAACA
2. What is the complementary DNA strand for: 5'-ACGCTCCACCTT-3'?
Solution:
Template: 5'-ACGCTCCACCTT-3'
Base pairing rules: A↔T, G↔C
A
→
T
→
C
→
G
→
G
→
C
→
C
→
G
→
T
→
A
→
C
→
G
→
C
→
G
→
A
→
T
→
C
→
G
→
C
→
G
→
T
→
A
→
T
→
A
Complement: 5'-TGCGAGGTGGAA-3'
Check: complement of complement = original (verified by code)
Answer: TGCGAGGTGGAA
3. What is the mRNA sequence transcribed from the template DNA strand: 3'-CTGCTACGCAAG-5'?
Solution:
Template DNA: 3'-CTGCTACGCAAG-5'
Transcription rules: A→U, T→A, G→C, C→G
C
→
G
→
T
→
A
→
G
→
C
→
C
→
G
→
T
→
A
→
A
→
U
→
C
→
G
→
G
→
C
→
C
→
G
→
A
→
U
→
A
→
U
→
G
→
C
mRNA: 5'-GACGAUGCGUUC-3'
Check: each base correctly transcribed (verified by code)
Answer: GACGAUGCGUUC
4. What is the mRNA sequence transcribed from the template DNA strand: 3'-CCAAGCTTGAGA-5'?
Solution:
Template DNA: 3'-CCAAGCTTGAGA-5'
Transcription rules: A→U, T→A, G→C, C→G
C
→
G
→
C
→
G
→
A
→
U
→
A
→
U
→
G
→
C
→
C
→
G
→
T
→
A
→
T
→
A
→
G
→
C
→
A
→
U
→
G
→
C
→
A
→
U
mRNA: 5'-GGUUCGAACUCU-3'
Check: each base correctly transcribed (verified by code)
Answer: GGUUCGAACUCU
Answers computed by code and double-checked by two independent methods.

Assumptions and Conventions

Assumptions:
  • The standard genetic code (NCBI transl_table=1) is used unless stated otherwise.
  • DNA sequences use the DNA alphabet (T, not U); mRNA uses U.
  • Translation starts at AUG (start codon) and stops at UAA, UAG, or UGA.
  • Reading frame: codons are read in groups of 3 from the start codon.
Conventions:
  • Course: biology
  • Difficulty: standard
Genetic code source: NCBI Taxonomy — The Standard Code (transl_table=1) (table 1), retrieved 2026-10-11.
Made with TeachSpark
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DNA, RNA and Protein - Biology - Transcription and translation

Complementary strands, transcription, translation, and mutation classification using the NCBI genetic code. No AI, no credits.

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